Anticorps Polyclonal de lapin anti-BMP5

BMP5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13253-1-AP

Synonymes

BMP 5, BMP5, BMP-5, bone morphogenetic protein 5

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Galerie de données de validation

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  WB analysis of mouse lung using 13253-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13253-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 13253-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HUVEC
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

13253-1-AP cible BMP5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMP5 Protéine recombinante Ag3952
• Nom complet: bone morphogenetic protein 5
• Masse moléculaire calculée: 454 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC027958
• Symbole du gène: BMP5
• Identification du gène (NCBI): 653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; G9a Knockdown Suppresses Cancer Aggressiveness by Facilitating Smad Protein Phosphorylation through Increasing BMP5 Expression in Luminal A Type Breast Cancer.; Authors - Yunho Jin
human	WB	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao
human	WB	Placenta; BMP5 promotes trophoblast functions upon N-glycosylation via the BMP5-SMAD1/5 signaling pathway in preeclampsia; Authors - Hao Wang
	WB	Phytomedicine; Lutein protects senescent ciliary muscle against oxidative stress through the Keap1/Nrf2/ARE pathway; Authors - Ning Gao
human	IHC	Mol Cancer; Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.; Authors - Erfei Chen

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