Anticorps Recombinant de lapin anti-BMP9

BMP9 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250512B9

N° de cat : 86411-1-PBS

Synonymes

BMP-9, Bone morphogenetic protein 9, Gdf2, GDF-2, Growth/differentiation factor 2

---

Galerie de données de validation

• 

  WB analysis using 86411-1-RR (same clone as 86411-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86411-1-RR (Gdf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86411-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02608-1

  The mean BMP9 concentration was determined to be 106.40 ng/mL in mouse liver tissue extract based on a 8.40 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02608-1

  Sandwich ELISA standard curve of MP02608-1, Mouse BMP9 Recombinant Matched Antibody Pair - PBS only. 86411-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2482. 86411-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

---

Informations sur le produit

86411-1-PBS cible BMP9 dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BMP9 Protéine recombinante Eg2482
• Nom complet: growth differentiation factor 2
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: NM_019506.4
• Symbole du gène: BMP9
• Identification du gène (NCBI): 12165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bone morphogenetic protein-9 (BMP9, also termed growth-differentiation factor-2, GDF-2) is a secreted signaling molecule that belongs to the transforming growth factor-β (TGF-β) super-family. First identified in fetal mouse liver cDNA libraries, BMP9 is predominantly produced by hepatic stellate cells and circulates at relatively high concentrations in adult plasma. BMP9 is synthesized as a precursor protein (pre-proBMP9) composed of 429 amino acids (aa) that includes a 22 aa signal peptide, a 297 aa prodomain (33 kDa) and a 110 aa mature protein (12.5 kDa). It is then cleaved by serine endoproteases, leading to a short dimeric mature form of 25 kDa, and the prodomain which can remain noncovalently associated with the mature short form, giving a size of about 100 kDa (PMID:21710321).

{{ptg:RelatedPrimaryAntibodies}}