Anticorps Polyclonal de lapin anti-BMSC UbP

BMSC UbP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11564-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 11564-1-AP (same clone as 11564-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 11564-1-AP (same clone as 11564-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11564-1-AP (same clone as 11564-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11564-1-AP (same clone as 11564-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11564-1-AP (same clone as 11564-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11564-1-AP (same clone as 11564-1-PBS)

  IP result of anti-BMSC UbP (IP:11564-1-AP, 4ug; Detection:11564-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11564-1-AP (same clone as 11564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11564-1-AP (BMSC UbP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11564-1-AP (same clone as 11564-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 11564-1-AP (BMSC UbP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11564-1-PBS cible BMSC UbP dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMSC UbP Protéine recombinante Ag2130
• Nom complet: ubiquitin-like 7 (bone marrow stromal cell-derived)
• Masse moléculaire calculée: 380 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC030055
• Symbole du gène: UBL7
• Identification du gène (NCBI): 84993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. UBL7 (ORF name SB132) encodes ubiquitin-like protein7 also named bone marrow stromal cell ubiquitin-like protein (BMSC-UbP) capable of binding ubiquitin. BMSC-UbP protein is derived from bone marrow stromal cells containing a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines and is decreased in BMSC stimulated with phorbol myristate acetate (PMA) suggesting a role in regulation of BMSC function.

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