Anticorps Polyclonal de lapin anti-BNIP1

BNIP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15964-1-AP

Synonymes

BNIP1, NIP1, SEC20, SEC20L, TRG 8

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Galerie de données de validation

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  WB analysis using 15964-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15964-1-AP (BNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15964-1-AP

  IP result of anti-BNIP1 (IP:15964-1-AP, 3ug; Detection:15964-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human heart using 15964-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15964-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

15964-1-AP cible BNIP1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BNIP1 Protéine recombinante Ag8737
• Nom complet: BCL2/adenovirus E1B 19kDa interacting protein 1
• Masse moléculaire calculée: 228 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC010959
• Symbole du gène: BNIP1
• Identification du gène (NCBI): 662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.

Publications

Species	Application	Title
human	WB,IP	Cell Signal; A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.; Authors - Wang Peng P
human	WB,IF	Hum Mutat; A homozygous hypomorphic BNIP1 variant causes an increase in autophagosomes and reduced autophagic flux and results in a spondylo-epiphyseal dysplasia.; Authors - Tess Holling
human	WB,IF	PLoS One; RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.; Authors - Tang Fei F
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss
mouse	IHC	Transl Oncol; Generation of a competing endogenous RNA network and validation of BNIP1 expression in the lung of irradiated mice; Authors - Qing-Hua Yu

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