Anticorps Polyclonal de lapin anti-BNIP1

BNIP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15964-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15964-1-AP (same clone as 15964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15964-1-AP (BNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 15964-1-AP (same clone as 15964-1-PBS)

  IP result of anti-BNIP1 (IP:15964-1-AP, 3ug; Detection:15964-1-AP 1:500) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15964-1-PBS cible BNIP1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BNIP1 Protéine recombinante Ag8737
• Nom complet: BCL2/adenovirus E1B 19kDa interacting protein 1
• Masse moléculaire calculée: 228 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC010959
• Symbole du gène: BNIP1
• Identification du gène (NCBI): 662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.

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