Anticorps Monoclonal anti-BNIP3

BNIP3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(33)

CloneNo.

2H7D8

Avis (2)

N° de cat : 68091-1-Ig

Synonymes

2H7D8, NIP3

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 68091-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68091-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 68091-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 68091-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68091-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68091-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 68091-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 68091-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 68091-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HSC-T6, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2 traitées au chlorure de cobalt,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 7 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

68091-1-Ig cible BNIP3 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BNIP3 Protéine recombinante Ag27935
• Nom complet: BCL2/adenovirus E1B 19kDa interacting protein 3
• Masse moléculaire calculée: 194 aa, 22 kDa
• Poids moléculaire observé: 25-30,50-60 kDa
• Numéro d’acquisition GenBank: BC009342
• Symbole du gène: BNIP3
• Identification du gène (NCBI): 664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells; Authors - Huafeng Zhang
mouse	WB,IHC,IF	Small; Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy; Authors - Dan Li
mouse	WB	Cell Biosci; Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy; Authors - Mei-Fei Wu
human	WB	Cell Rep; Proline restores mitochondrial function and reverses aging hallmarks in senescent cells; Authors - Debanik Choudhury
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
mouse	WB,IHC	Cell Commun Signal; Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1; Authors - Xudong Xie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paula (Verified Customer) (07-26-2024)

Worked well for Western Blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Bone Marrow Mesenchymal Stem Cells

FH

Aidan (Verified Customer) (04-10-2024)

worked v well, would highly recommend. Incubated overnight 4 degrees 1:5000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse muller

BNIP3 Antibody Western Blot validation (1:5000 dilution) in Mouse muller (Cat no:68091-1-Ig)

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