Anticorps Monoclonal anti-BNIP3

BNIP3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H7D8

N° de cat : 68091-1-PBS

Synonymes

2H7D8, NIP3

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 68091-1-Ig (same clone as 68091-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68091-1-Ig (same clone as 68091-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68091-1-PBS cible BNIP3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BNIP3 Protéine recombinante Ag27935
• Nom complet: BCL2/adenovirus E1B 19kDa interacting protein 3
• Masse moléculaire calculée: 194 aa, 22 kDa
• Poids moléculaire observé: 25-30,50-60 kDa
• Numéro d’acquisition GenBank: BC009342
• Symbole du gène: BNIP3
• Identification du gène (NCBI): 664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

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