Anticorps Monoclonal anti-BNIP3L

BNIP3L Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6A3

N° de cat : 68118-1-PBS

Synonymes

BNIP3H, BNIP3A, BNIP, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, Adenovirus E1B19K-binding protein B5

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Galerie de données de validation

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  WB analysis using 68118-1-Ig (same clone as 68118-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68118-1-Ig (same clone as 68118-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68118-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68118-1-PBS cible BNIP3L dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BNIP3L Protéine recombinante Ag31547
• Nom complet: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 35 kD
• Numéro d’acquisition GenBank: BC001559
• Symbole du gène: BNIP3L
• Identification du gène (NCBI): 665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BNIP3L/Nix is a mitochondrial protein from the outer membrane that belongs to the BH3-only protein from the BCL2 family. BNIP3L was initially recognized as a proapoptotic protein with milder efficacy in inducing apoptosis compared to other proteins in this family. These phenotypes raised concerns about the molecular function of BNIP3L besides inducing apoptosis. Moreover, studies have shown that BNIP3L is a 24-kDa protein, which is predominantly expressed as a 48-kDa dimer. When analyzed by SDS-PAGE, BNIP3 migrates predominantly as a about 60 kDa dimer in addition to the 30 kDa monomer. (PMID: 34930907, PMID: 32286918)

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