Anticorps Recombinant de lapin anti-BOP1

• Phare
• Validé par KD/KO

BOP1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A4

N° de cat : 82742-3-PBS

Synonymes

KIAA0124, block of proliferation 1, 3A4

---

Galerie de données de validation

• 

  WB analysis using 82742-3-RR (same clone as 82742-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

  WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82742-3-RR (same clone as 82742-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

---

Informations sur le produit

82742-3-PBS cible BOP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BOP1 Protéine recombinante Ag26669
• Nom complet: block of proliferation 1
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC013787
• Symbole du gène: BOP1
• Identification du gène (NCBI): 23246
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).

{{ptg:RelatedPrimaryAntibodies}}