Anticorps Monoclonal anti-BORIS

BORIS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B12E2

N° de cat : 60079-1-PBS

Synonymes

CTCFL, 2B12E2, CCCTC-binding factor, CT27, CTCF paralog

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Galerie de données de validation

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  WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 60079-1-Ig (same clone as 60079-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60079-1-PBS cible BORIS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BORIS Protéine recombinante Ag1003
• Nom complet: CCCTC-binding factor (zinc finger protein)-like
• Masse moléculaire calculée: 82 kDa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC130486
• Symbole du gène: BORIS/CTCFL
• Identification du gène (NCBI): 140690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

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