Anticorps Polyclonal de lapin anti-BOULE

BOULE Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13720-1-PBS

Synonymes

BOLL, Protein boule-like

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Galerie de données de validation

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  WB analysis using 13720-1-AP (same clone as 13720-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 13720-1-AP (BOULE antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13720-1-PBS cible BOULE dans les applications de WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BOULE Protéine recombinante Ag4662
• Nom complet: bol, boule-like (Drosophila)
• Masse moléculaire calculée: 283 aa, 31 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC033674
• Symbole du gène: BOULE
• Identification du gène (NCBI): 66037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

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