Anticorps Polyclonal de lapin anti-BOULE

BOULE Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-13720

Synonymes

bol, boule like (Drosophila), BOLL, BOULE, Protein boule like

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Galerie de données de validation

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  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (cold ethanol) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:100, CoraLite®555 TNP1 antibody (CL555-17178, orange), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

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Applications testées

• Résultats positifs en IF: tissu testiculaire de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-13720 cible BOULE dans les applications de IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BOULE Protéine recombinante Ag4662
• Nom complet: bol, boule-like (Drosophila)
• Masse moléculaire calculée: 283 aa, 31 kDa
• Numéro d’acquisition GenBank: BC033674
• Symbole du gène: BOLL
• Identification du gène (NCBI): 66037
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).