Anticorps Polyclonal de lapin anti-BPIL1

BPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13461-2-AP

Synonymes

Bactericidal/permeability-increasing protein-like 1, BPI fold-containing family B member 2, BPIFB2, BPI-like 1, C20orf184

---

Galerie de données de validation

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13461-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13461-2-AP (BPIL1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.

• 

  IF Staining of A549 using 13461-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using BPIL1 antibody (13461-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

13461-2-AP cible BPIL1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BPIL1 Protéine recombinante Ag4051
• Nom complet: bactericidal/permeability-increasing protein-like 1
• Masse moléculaire calculée: 458 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC034415
• Symbole du gène: BPIL1
• Identification du gène (NCBI): 80341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bactericidal/permeability-increasing protein-like 1 (BPIL1, also known as LPLUNC2 or BPIFB2) is a member of the lipid transfer (LT)/lipopolysaccharide binding protein (LBP) family. LT/LBP proteins are structurally related proteins capable of binding phospholipids and lipopolysaccharides (PMID: 12185532). The gene of BPIL1 maps to Chromosome 20q11, and encodes a 458-amino acid protein with a calculated molecular weight of 49 kDa. It is highly expressed in hypertrophic tonsils. BPIL1 has been identified as a nasal mucous protein that may be involved in immune response in the nose against microbial infections (PMID: 15996010). Reduced expression of antimicrobial PLUNC proteins (LPLUNC1 and LPLUNC2) has been reported in nasal polyp tissues of patients with chronic rhinosinusitis (PMID: 22676062).

Publications

Species	Application	Title
human	WB,IHC	Allergy; Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.; Authors - Seshadri S S
human	WB	J Proteome Res; Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.; Authors - Foster Matthew W MW
human	IHC	J Immunol Res; Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.; Authors - Yanran Huang

{{ptg:RelatedPrimaryAntibodies}}