Anticorps Recombinant de lapin anti-BRAP

BRAP Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240099D8

N° de cat : 83314-6-PBS

Synonymes

Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein

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Galerie de données de validation

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  IF Staining of MCF-7 using 83314-6-RR (same clone as 83314-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83314-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83314-6-RR (same clone as 83314-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83314-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83314-6-RR (same clone as 83314-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BRAP Recombinant antibody (83314-6-RR, Clone:240099D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83314-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83314-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83314-6-RR against Human BRAP were performed. The affinity constant is 6.34 nM.

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Informations sur le produit

83314-6-PBS cible BRAP dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BRAP Protéine recombinante Ag26728
• Nom complet: BRCA1 associated protein
• Masse moléculaire calculée: 592 aa, 67 kDa
• Numéro d’acquisition GenBank: BC136698
• Symbole du gène: BRAP
• Identification du gène (NCBI): 8315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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