Anticorps Monoclonal anti-BRCA1

BRCA1 Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF,ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

20D7G6

N° de cat : 66267-1-Ig

Synonymes

BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53

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Galerie de données de validation

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (BRCA1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (BRCA1 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

66267-1-Ig cible BRCA1 dans les applications de IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BRCA1 Protéine recombinante Ag19178
• Nom complet: breast cancer 1, early onset
• Masse moléculaire calculée: 1863 aa, 208 kDa
• Poids moléculaire observé: 207 kDa
• Numéro d’acquisition GenBank: BC115037
• Symbole du gène: BRCA1
• Identification du gène (NCBI): 672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Publications

Species	Application	Title
human	IF	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu