Anticorps Recombinant de lapin anti-BRCC3

BRCC3 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242931B9

N° de cat : 85430-4-PBS

Synonymes

BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36

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Galerie de données de validation

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  WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  IP experiment of SK-BR-3 using 85430-4-RR (same clone as 85430-4-PBS)

  IP result of anti-BRCC3 (IP:85430-4-RR, 4ug; Detection:85430-4-RR 1:2000) with SK-BR-3 cells lysate 1280 ug. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 85430-4-RR (same clone as 85430-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  IHC staining of Human placenta using 85430-4-RR (same clone as 85430-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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Informations sur le produit

85430-4-PBS cible BRCC3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BRCC3 Protéine recombinante Ag7584
• Nom complet: BRCA1/BRCA2-containing complex, subunit 3
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002999
• Symbole du gène: BRCC3
• Identification du gène (NCBI): 79184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)

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