Anticorps Recombinant de lapin anti-BRD2

BRD2 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

250453G5

N° de cat : 85990-2-RR

Synonymes

bromodomain containing 2, Bromodomain-containing protein 2, D6S113E, FSH, FSRG1

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Galerie de données de validation

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  WB analysis using 85990-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85990-2-RR (BRD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 85990-2-RR

  IP result of anti-BRD2 (IP:85990-2-RR, 4ug; Detection:85990-2-RR 1:3000) with HeLa cells lysate 1600 ug.

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  IHC staining of mouse colon using 85990-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85990-2-RR (BRD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 85990-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BRD2 antibody (85990-2-RR, Clone: 250453G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules C2C12, cellules Jurkat, cellules K-562, cellules PC-12
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85990-2-RR cible BRD2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BRD2 Protéine recombinante Ag17589
• Nom complet: bromodomain containing 2
• Masse moléculaire calculée: 836 aa, 92 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC063840
• Symbole du gène: BRD2
• Identification du gène (NCBI): 6046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD2 (Bromodomain-containing protein 2), also known as RING3, is a member of the Bromodomain and Extra-Terminal (BET) family of proteins. These proteins contain two bromodomains and one extra-terminal domain. BRD2 primarily localizes to the nucleus and functions as an atypical protein kinase. Its main role involves the activation of E2F1 and E2F2 proteins, facilitating the production of proteins essential for the G1/S phase transition of the cell cycle. BRD2 specifically recognizes acetylated histones through its bromodomains, particularly acetylated lysine residues on histones H3 and H4. This interaction allows BRD2 to recruit various transcription factors, co-activators, and repressors, thereby modulating transcriptional activity. BRD2 plays a crucial role in chromatin remodeling by recruiting proteins such as histone deacetylases. It is highly expressed during neural tube development and closure, making it essential for neurogenesis and embryogenesis.(PMID: 33462181, PMID: 37926722)

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