Anticorps Polyclonal de lapin anti-BRD4

• Phare
• Validé par KD/KO

BRD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 28486-1-AP

Synonymes

Protein HUNK1, MCAP, HUNKI, Bromodomain-containing protein 4, bromodomain containing 4

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Galerie de données de validation

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  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 28486-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 28486-1-AP

  IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug.

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  IHC staining of human lung cancer using 28486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (28486-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231, cellules Ramos, cellules T-47D
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

28486-1-AP cible BRD4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bromodomain containing 4
• Masse moléculaire calculée: 1362 aa, 152 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC035266
• Symbole du gène: BRD4
• Identification du gène (NCBI): 23476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. The molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).

Publications

Species	Application	Title
	WB	Sci Rep; BRD4 as the key lactylation related gene in heart failure identified through bioinformatics analysis; Authors - Kaiyuan Li
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human,mouse	WB,CoIP	Biochem Pharmacol; BRD4 promotes hepatic stellate cells activation and hepatic fibrosis via mediating P300/H3K27ac/PLK1 axis; Authors - Miao Cheng ; KD Validated
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang
human	WB	J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan
human	WB	Front Med (Lausanne); Screening and identification of microRNAs mediating cartilage endplate degeneration in human intervertebral disks; Authors - Bei Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (06-13-2024)

detects 3 BRD4 bands in HeLa cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell line

BRD4 Antibody Western Blot validation (1:1000 dilution) in HeLa cell line (Cat no:28486-1-AP)

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