Anticorps Polyclonal de lapin anti-BRD4

BRD4 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 28486-1-AP

Synonymes

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

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Galerie de données de validation

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  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 28486-1-AP

  IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231, cellules Ramos, cellules T-47D
• Résultats positifs en IP: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

28486-1-AP cible BRD4 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bromodomain containing 4
• Masse moléculaire calculée: 1362 aa, 152 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC035266
• Symbole du gène: BRD4
• Identification du gène (NCBI): 23476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members.

Publications

Species	Application	Title
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human,mouse	WB,CoIP	Biochem Pharmacol; BRD4 promotes hepatic stellate cells activation and hepatic fibrosis via mediating P300/H3K27ac/PLK1 axis; Authors - Miao Cheng ; KD Validated
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang