Anticorps Polyclonal de lapin anti-BRD4

• Phare
• Validé par KD/KO

BRD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28486-1-PBS

Synonymes

Protein HUNK1, MCAP, HUNKI, Bromodomain-containing protein 4, bromodomain containing 4

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Galerie de données de validation

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  WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 28486-1-AP (same clone as 28486-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 28486-1-AP (same clone as 28486-1-PBS)

  IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28486-1-AP (same clone as 28486-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (28486-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28486-1-PBS cible BRD4 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bromodomain containing 4
• Masse moléculaire calculée: 1362 aa, 152 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC035266
• Symbole du gène: BRD4
• Identification du gène (NCBI): 23476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. The molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).

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