Anticorps Monoclonal anti-BRD4

BRD4 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, ChIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2F7E2

N° de cat : 67374-2-Ig

Synonymes

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

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Galerie de données de validation

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  WB analysis using 67374-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67374-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• ChIP: See 2 publications below

Informations sur le produit

67374-2-Ig cible BRD4 dans les applications de WB, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BRD4 Protéine recombinante Ag27545
• Nom complet: bromodomain containing 4
• Masse moléculaire calculée: 1362 aa, 152 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC035266
• Symbole du gène: BRD4
• Identification du gène (NCBI): 23476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Bioengineered; Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p.; Authors - Dan-Dan Zhu
human	WB,ChIP	Curr Med Sci; ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer; Authors - Yang-Kai Jiang
human		J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan
mouse	WB	Nat Commun; MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition; Authors - Fan Tong
human,rat	WB	Tissue Cell; Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization; Authors - Lei Zhu ; KD Validated
human	ChIP	Cell; De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses; Authors - Xiao-Qi Liu

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