Anticorps Recombinant de lapin anti-BRD7

BRD7 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250776B2

N° de cat : 86176-2-RR

Synonymes

BP75, Bromodomain-containing protein 7, CELTIX1, NAG4, Protein CELTIX 1

---

Galerie de données de validation

• 

  WB analysis using 86176-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 86176-2-RR

  PC-3 cells were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 86176-2-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules COLO 320, cellules PC-3, cellules SH-SY5Y, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86176-2-RR cible BRD7 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BRD7 Protéine recombinante Ag1461
• Nom complet: bromodomain containing 7
• Masse moléculaire calculée: 651 aa, 74 kDa
• Poids moléculaire observé: 74-78 kDa
• Numéro d’acquisition GenBank: BC050728
• Symbole du gène: BRD7
• Identification du gène (NCBI): 29117
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.

{{ptg:RelatedPrimaryAntibodies}}