Anticorps Monoclonal anti-BRD8

• Phare
• Validé par KD/KO

BRD8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5B6D6

N° de cat : 60121-1-PBS

Synonymes

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

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Galerie de données de validation

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  WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

  WB result of BRD8 antibody (60121-1-Ig, 1:8000) with si-Control and si-BRD8 transfected HeLa cells.. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-Ig (BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HEK-293 cells using 60121-1-Ig(BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD8 antibody (60121-1-Ig, Clone: 5B6D6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60121-1-PBS cible BRD8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BRD8 Protéine recombinante Ag0790
• Nom complet: bromodomain containing 8
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC008039
• Symbole du gène: BRD8
• Identification du gène (NCBI): 10902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].

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