Anticorps Recombinant de lapin anti-BRD8

BRD8 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242610F5

N° de cat : 84965-1-RR

Synonymes

242610F5, bromodomain containing 8, Bromodomain-containing protein 8, p120, SMAP

---

Galerie de données de validation

• 

  WB analysis using 84965-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84965-1-RR (BRD8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 84965-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84965-1-RR (BRD8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 84965-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84965-1-RR (BRD8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 84965-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody (84965-1-RR, Clone: 242610F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84965-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84965-1-RR against Human BRD8 were performed. The affinity constant is 0.645 nM.

---

Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293T, cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84965-1-RR cible BRD8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BRD8 Protéine recombinante Ag0790
• Nom complet: bromodomain containing 8
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 120-170 kDa
• Numéro d’acquisition GenBank: BC008039
• Symbole du gène: BRD8
• Identification du gène (NCBI): 10902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)

{{ptg:RelatedPrimaryAntibodies}}