Anticorps Monoclonal anti-BRE

BRE Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5A5

N° de cat : 66356-1-PBS

Synonymes

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

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Galerie de données de validation

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  WB analysis of fetal human brain using 66356-1-Ig (same clone as 66356-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66356-1-Ig (same clone as 66356-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66356-1-Ig (same clone as 66356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66356-1-Ig (same clone as 66356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66356-1-Ig (same clone as 66356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66356-1-Ig (same clone as 66356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66356-1-Ig (same clone as 66356-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRE antibody (66356-1-Ig, Clone: 2E5A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66356-1-PBS cible BRE dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BRE Protéine recombinante Ag2256
• Nom complet: brain and reproductive organ-expressed (TNFRSF1A modulator)
• Masse moléculaire calculée: 383 aa, 44 kDa
• Poids moléculaire observé: 42-47 kDa
• Numéro d’acquisition GenBank: BC001251
• Symbole du gène: BRE
• Identification du gène (NCBI): 9577
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

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