Anticorps Polyclonal de lapin anti-BRPF1

BRPF1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30186-1-PBS

Synonymes

Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140

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Galerie de données de validation

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  WB analysis using 30186-1-AP (same clone as 30186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30186-1-AP (BRPF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRPF1 antibody (30186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human BRPF1 (30186-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30186-1-PBS cible BRPF1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRPF1 Protéine recombinante Ag32828
• Nom complet: bromodomain and PHD finger containing, 1
• Masse moléculaire calculée: 1220 aa, 138 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC053851
• Symbole du gène: BRPF1
• Identification du gène (NCBI): 7862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

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