Anticorps Recombinant de lapin anti-BRSK1

BRSK1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

251341A11

N° de cat : 86462-1-PBS

Synonymes

BR serine/threonine kinase 1, BR serine/threonine-protein kinase 1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 86462-1-RR (same clone as 86462-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86462-1-RR (BRSK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86462-1-PBS in a different storage buffer formulation.

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  Sample test of MP02463-1

  The mean BRSK1 concentration was determined to be 8.4 ng/mL in Mouse brain tissue extract based on a 7.0 mg/mL extract load.

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  IHC staining of mouse stomach using 86462-1-RR (same clone as 86462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86462-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 86462-1-RR (same clone as 86462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86462-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 86462-1-RR (same clone as 86462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86462-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02463-1

  Cytometric bead array standard curve of MP02463-1, BRSK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86462-2-PBS. Detection antibody: 86462-1-PBS. Standard: Ag3357. Range: 0.312-40 ng/mL.

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  Affinity and Kinetic Characterization of 86462-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86462-1-RR against Human BRSK1 were performed. The affinity constant is 0.173 nM.

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Informations sur le produit

86462-1-PBS cible BRSK1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BRSK1 Protéine recombinante Ag3357
• Nom complet: BR serine/threonine kinase 1
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC016681
• Symbole du gène: BRSK1
• Identification du gène (NCBI): 84446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.

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