Anticorps Polyclonal de lapin anti-BRWD1

BRWD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29293-1-PBS

Synonymes

Bromodomain and WD repeat-containing protein 1, C21orf107, N143, WD repeat containing protein 9, WD repeat-containing protein 9

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Galerie de données de validation

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  WB analysis using 29293-1-AP (same clone as 29293-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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  WB analysis using 29293-1-AP (same clone as 29293-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29293-1-AP (same clone as 29293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29293-1-AP (same clone as 29293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29293-1-PBS cible BRWD1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRWD1 Protéine recombinante Ag30902
• Nom complet: bromodomain and WD repeat domain containing 1
• Masse moléculaire calculée: 2320 aa, 263 kDa
• Poids moléculaire observé: 263 kDa
• Numéro d’acquisition GenBank: BC064602
• Symbole du gène: BRWD1
• Identification du gène (NCBI): 54014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BRWD1 (Bromodomain and WD repeat domain containing 1, also known as WDR9) has a predicted molecular weight of 263 kDa and contains tandem BROMO domains and WD40 repeats (PMID:12889071). One of the remarkable functions of BRWD1 was the coordinated repression of MYC and MYC target genes. At the Myc/MYC locus, in both mice and humans, BRWD1 specififically repressed distal enhancers, which are associated with lineage specifific expression (PMID:30250168). In addition,BRWD1 as a key epigenomic mediator of normal neurodevelopment and an important contributor to DS (Down syndrome)-related phenotypes (PMID:36289231).

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