Anticorps Polyclonal de lapin anti-BSAP,PAX5

• Phare
• Validé par KD/KO

BSAP,PAX5 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 26709-1-AP

Synonymes

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

---

Galerie de données de validation

• 

  WB analysis using 26709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 26709-1-AP

  WB result of BSAP,PAX5 antibody (26709-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BSAP,PAX5 transfected Raji cells.

• 

  WB analysis of Raji using 26709-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 26709-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Ramos using 26709-1-AP

  IP result of anti-BSAP,PAX5 (IP:26709-1-AP, 4ug; Detection:26709-1-AP 1:1000) with Ramos cells lysate 3600 ug.

• 

  IHC staining of human lymphoma using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Ramos using 26709-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.20 ug/test Anti-Human BSAP,PAX5 (26709-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos
• Résultats positifs en IP: cellules Ramos,
• Résultats positifs en IHC: tissu de lymphome humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below

Informations sur le produit

26709-1-AP cible BSAP,PAX5 dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BSAP,PAX5 Protéine recombinante Ag25147
• Nom complet: paired box 5
• Masse moléculaire calculée: 391 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC156927
• Symbole du gène: PAX5
• Identification du gène (NCBI): 5079
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
human	IHC	J Cutan Pathol; Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.; Authors - Jennifer S Ko
mouse	IHC	Mol Oncol; miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer; Authors - Shuai Wu