Anticorps Polyclonal de lapin anti-BST2

• Phare
• Validé par KD/KO

BST2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 13560-1-AP

Synonymes

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

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Galerie de données de validation

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  WB analysis using 13560-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13560-1-AP (BST2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13560-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13560-1-AP (BST2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13560-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BST2 antibody (13560-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 13560-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug BST2 antibody (13560-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13560-1-AP cible BST2 dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BST2 Protéine recombinante Ag4430
• Nom complet: bone marrow stromal cell antigen 2
• Masse moléculaire calculée: 180 aa, 20 kDa
• Poids moléculaire observé: 30-36 kDa, 50-70 kDa
• Numéro d’acquisition GenBank: BC033873
• Symbole du gène: BST2
• Identification du gène (NCBI): 684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Publications

Species	Application	Title
human	WB	Mol Cell; PMID: 28965816; Authors - Shouheng Jin ; KD Validated
human	WB	Proc Natl Acad Sci U S A; PMID: 24753610; Authors - Xuesen Zhao
human	WB, IF	Curr Biol; The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.; Authors - Adrien Presle ; KO Validated
human	WB	PLoS Pathog; PMID: 28910400; Authors - Yuxuan Fu ; KO Validated
human	WB, IF	J Virol; Interferon-inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion.; Authors - Ran Yan ; KD Validated
human	WB	J Virol; Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.; Authors - Weidner Jessica M JM

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (10-08-2021)

Detects endogenous BST2 in HeLa cells (BST2 positive), also when overexpressed as FLAG tagged version in HeLa cells. Does no detect anything in HEK293T cells (BST2 negative) but detects many bands when FLAG BST2 or untagged BST2 is overexpressed in these cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell culture of HeLa and HEK293T

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