Anticorps Polyclonal de lapin anti-BTD

BTD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16330-1-PBS

Synonymes

EC:3.5.1.12, biotinidase, Biotinase

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Galerie de données de validation

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  WB analysis using 16330-1-AP (same clone as 16330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16330-1-AP (BTD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 16330-1-AP (same clone as 16330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16330-1-AP (same clone as 16330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTD antibody (16330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16330-1-PBS cible BTD dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BTD Protéine recombinante Ag9449
• Nom complet: biotinidase
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC012099
• Symbole du gène: BTD
• Identification du gène (NCBI): 686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.

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