Anticorps Recombinant de lapin anti-BTD

BTD Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242057C3

N° de cat : 84613-2-PBS

Synonymes

Biotinase, biotinidase, EC:3.5.1.12

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Galerie de données de validation

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  WB analysis using 84613-2-RR (same clone as 84613-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84613-2-RR (BTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84613-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01452-1

  Cytometric bead array standard curve of MP01452-1, BTD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84613-1-PBS. Detection antibody: 84613-2-PBS. Standard: Ag9449. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84613-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84613-2-PBS against Human BTD was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 84613-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84613-2-RR against Human BTD were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84613-2-PBS cible BTD dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BTD Protéine recombinante Ag9449
• Nom complet: biotinidase
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC012099
• Symbole du gène: BTD
• Identification du gène (NCBI): 686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in the regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.

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