Anticorps Recombinant de lapin anti-BTLA/CD272

BTLA/CD272 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242133E5

N° de cat : 84754-5-RR

Synonymes

242133E5, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, Btla, CD272

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Galerie de données de validation

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  WB analysis using 84754-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84754-5-RR (BTLA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A20 using 84754-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using BTLA antibody (84754-5-RR, Clone: 242133E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84754-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84754-5-RR against Mouse BTLA were performed. The affinity constant is 1.12 nM.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu de thymus de souris
• Résultats positifs en IF/ICC: A20 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84754-5-RR cible BTLA/CD272 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BTLA/CD272 Protéine recombinante Eg1843
• Nom complet: B and T lymphocyte associated
• Masse moléculaire calculée: 34kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: AAI08964.1
• Symbole du gène: Btla
• Identification du gène (NCBI): 208154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BTLA, or B and T lymphocyte attenuator, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

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