Anticorps Recombinant de lapin anti-BTLA/CD272

BTLA/CD272 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242133A1

N° de cat : 98246-2-PBS

Synonymes

CD272, Btla, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133A1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98246-2-RR (same clone as 98246-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed. This data was developed using the same antibody clone with 98246-2-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98246-2-RR (same clone as 98246-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98246-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98246-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98246-2-RR against Mouse BTLA/CD272 were performed. The affinity constant is 0.441 nM.

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Informations sur le produit

98246-2-PBS cible BTLA/CD272 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: B and T lymphocyte associated
• Masse moléculaire calculée: 34kDa
• Numéro d’acquisition GenBank: AAI08964.1
• Symbole du gène: Btla
• Identification du gène (NCBI): 208154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

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