Anticorps Recombinant de lapin anti-BTLA/CD272

BTLA/CD272 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241318H5

N° de cat : CL647-98096

Synonymes

BTLA, 241318H5, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using CL647-98096

  1x10^6 human PBMCs were surface stained with PE Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (PE-65569, Clone: OKT3), and 5 ul CoraLite® Plus 647 Anti-Human BTLA/CD272 Rabbit RecAb (CL647-98096, Clone: 241318H5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98096

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human BTLA/CD272 Rabbit RecAb (CL647-98096, Clone:241318H5)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98096 cible BTLA/CD272 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: B and T lymphocyte associated
• Masse moléculaire calculée: 289 aa, 33 kDa
• Numéro d’acquisition GenBank: BC107091
• Symbole du gène: BTLA
• Identification du gène (NCBI): 151888
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

{{ptg:RelatedPrimaryAntibodies}}