Anticorps Recombinant de lapin anti-BTLA/CD272

BTLA/CD272 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

242133A1

N° de cat : CL647-98246-2

Synonymes

242133A1, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, Btla, CD272

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL647-98246-2

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2) (CL488-65139, Clone: RA3-6B2), and 0.25 ug CoraLite® Plus 647 Anti-Mouse BTLA/CD272 Rabbit RecAb (CL647-98246-2, Clone: 242133A1) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-98246-2

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse BTLA/CD272 Rabbit RecAb (CL647-98246-2, Clone: 242133A1) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98246-2 cible BTLA/CD272 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BTLA/CD272 Protéine recombinante Eg1843
• Nom complet: B and T lymphocyte associated
• Masse moléculaire calculée: 34kDa
• Numéro d’acquisition GenBank: AAI08964.1
• Symbole du gène: Btla
• Identification du gène (NCBI): 208154
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

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