Anticorps Polyclonal de lapin anti-BTN2A2

BTN2A2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16482-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of MCF-7 using 16482-1-AP (same clone as 16482-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 16482-1-AP (same clone as 16482-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16482-1-AP (same clone as 16482-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16482-1-PBS cible BTN2A2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BTN2A2 Protéine recombinante Ag9603
• Nom complet: butyrophilin, subfamily 2, member A2
• Masse moléculaire calculée: 523 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC014021
• Symbole du gène: BTN2A2
• Identification du gène (NCBI): 10385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).

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