Anticorps Polyclonal de lapin anti-BTN3A1

• Phare
• Validé par KD/KO

BTN3A1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 25221-1-AP

Synonymes

CD277, BTF5, BT3.1

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Galerie de données de validation

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  WB analysis of U-937 using 25221-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 25221-1-AP (BTN3A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25221-1-AP (BTN3A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 25221-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BTN3A1 antibody (25221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-937, cellules Jurkat
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

25221-1-AP cible BTN3A1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BTN3A1 Protéine recombinante Ag18414
• Nom complet: butyrophilin, subfamily 3, member A1
• Masse moléculaire calculée: 513 aa, 58 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC121800
• Symbole du gène: BTN3A1
• Identification du gène (NCBI): 11119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BTN3A1, also named as Butyrophilin subfamily 3 member A1 or BTF5, is a 513 amino acid protein ,which contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 localizes in the cell membrane and belongs to the immunoglobulin superfamily. BTN/MOG family. BTN3A1 is detected on T-cells, natural killer cells, dendritic cells and macrophages. It is ubiquitously highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. It also regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. It exists as four isoforms. We detected a 48 kda isoform by western blot.

Publications

Species	Application	Title
human	WB	Nat Commun; The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.; Authors - Barbara Castella ; KD Validated
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human	WB	Biochem Biophys Res Commun; Expression level of BTN3A1 on the surface of CD14+ monocytes is a potential predictor of γδ T cell expansion efficiency.; Authors - Mako Tomogane
human	WB, IHC	FEBS Open Bio; Comprehensive analysis of BTN3A1 in cancers: mining of omics data and validation in patient samples and cellular models.; Authors - Fan Liang

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