Anticorps Polyclonal de lapin anti-BUB1

• Phare
• Validé par KD/KO

BUB1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13330-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13330-1-AP (same clone as 13330-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 13330-1-AP (same clone as 13330-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

  IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 13330-1-AP (same clone as 13330-1-PBS)

  Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13330-1-PBS cible BUB1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BUB1 Protéine recombinante Ag4138
• Nom complet: budding uninhibited by benzimidazoles 1 homolog (yeast)
• Masse moléculaire calculée: 1085 aa, 122 kDa
• Poids moléculaire observé: 45 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC028201
• Symbole du gène: BUB1
• Identification du gène (NCBI): 699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

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