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Anticorps Recombinant de lapin anti-BUB1

BUB1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251580F8

N° de cat : 86843-1-PBS

Synonymes

BUB1A, BUB1L, EC:2.7.11.1, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using BUB1 Recombinant monoclonal antibody (86843-1-RR)

    WB analysis using 86843-1-RR (same clone as 86843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86843-1-RR (BUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86843-1-PBS in a different storage buffer formulation.

Informations sur le produit

86843-1-PBS cible BUB1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène BUB1 Protéine recombinante Ag4138
Nom complet budding uninhibited by benzimidazoles 1 homolog (yeast)
Masse moléculaire calculée 1085 aa, 122 kDa
Poids moléculaire observé120 kDa
Numéro d’acquisition GenBankBC028201
Symbole du gène BUB1
Identification du gène (NCBI) 699
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

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