Anticorps Monoclonal anti-BUB1B

BUB1B Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3B2D5

N° de cat : 60423-1-Ig

Synonymes

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

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Galerie de données de validation

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  WB analysis using 60423-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60423-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HaCaT, cellules HEK-293, cellules HeLa, cellules Ramos, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60423-1-Ig cible BUB1B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BUB1B Protéine recombinante Ag34182
• Nom complet: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Masse moléculaire calculée: 1050 aa, 120 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC018739
• Symbole du gène: BubR1
• Identification du gène (NCBI): 701
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

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