Anticorps Polyclonal de lapin anti-BUD31

• Phare
• Validé par KD/KO

BUD31 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11798-1-AP

Synonymes

BUD31, BUD31 homolog (S. cerevisiae), EDG 2, EDG2, fSAP17, G10, Protein BUD31 homolog, Protein EDG 2, Protein G10 homolog, YCR063W

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Galerie de données de validation

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  WB analysis of mouse brain using 11798-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11798-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of hESC using 11798-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11798-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 11798-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11798-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11798-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11798-1-AP

  IP Result of anti-BUD31 (IP:11798-1-AP, 4ug; Detection:11798-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human colon cancer using 11798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11798-1-AP (BUD31 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Jurkat, cellules Raji, cellules SCEh, tissu cardiaque de souris, tissu cérébral humain, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

11798-1-AP cible BUD31 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BUD31 Protéine recombinante Ag2373
• Nom complet: BUD31 homolog (S. cerevisiae)
• Masse moléculaire calculée: 144 aa, 17 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC022821
• Symbole du gène: BUD31
• Identification du gène (NCBI): 8896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; The spliceosome is a therapeutic vulnerability in MYC-driven cancer.; Authors - Tiffany Y-T Hsu ; KD Validated
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
mouse	WB,RIP	Cell Death Differ; Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation; Authors - Junchao Qin ; KD Validated
human	IHC,IF,CoIP,RIP	Nat Commun; Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12; Authors - Zixiang Wang ; KD Validated