Anticorps Recombinant de lapin anti-BZW1

BZW1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243162H4

N° de cat : 85655-1-PBS

Synonymes

5MP2, Basic leucine zipper and W2 domain-containing protein 1, BZAP45, eIF5-mimic protein 2, KIAA0005

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Galerie de données de validation

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  WB analysis using 85655-1-RR (same clone as 85655-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85655-1-RR (BZW1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85655-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 85655-1-RR (same clone as 85655-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using BZW1 antibody (85655-1-RR, Clone: 243162H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85655-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02022-1

  Cytometric bead array standard curve of MP02022-1, BZW1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85655-3-PBS. Detection antibody: 85655-1-PBS. Standard: Ag13830. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85655-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85655-1-RR against Human BZW1 were performed. The affinity constant is 1.62 nM.

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Informations sur le produit

85655-1-PBS cible BZW1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BZW1 Protéine recombinante Ag13830
• Nom complet: basic leucine zipper and W2 domains 1
• Masse moléculaire calculée: 353 aa, 41 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC001804
• Symbole du gène: BZW1
• Identification du gène (NCBI): 9689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BZW1, also known as basic leucine zipper and W2 domains 1, is a member of the basic leucine zipper (bZIP) superfamily of transcription factors. It is a 45 kDa protein that contains an N-terminal bZIP domain for protein interactions and a C-terminal nucleotide (ATP or GTP) binding domain. Human BZW1 can activate transcription of the histone H4 gene and serve as a co-regulator with other transcription factors to control the cell cycle. In recent years, BZW1 has been identified as enhancing phosphorylation to promote glycolysis in pancreatic ductal adenocarcinoma. Moreover, BZW1 has been found to regulate the cell cycle in ovarian cancer, thereby promoting its progression. Additionally, BZW1 plays a crucial role in mucoepidermoid carcinoma of the salivary glands. BZW1 is also involved in the regulation of translation initiation, acting as a translational rheostat and autoregulating its own translation. It has been suggested that BZW1, as well as its paralog BZW2, is an eIF5-mimic protein. BZW1 has been shown to facilitate glycolysis and promote tumor growth in pancreatic ductal adenocarcinoma through potentiating eIF2α phosphorylation, and it may serve as a therapeutic target for patients with pancreatic cancer. In macrophages, activation of BZW1 by CEBPB promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress. BZW1 has also been found to be associated with the Wnt/β-catenin pathway in lung adenocarcinoma, potentially influencing epithelial-mesenchymal transition (EMT) processes.

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