Anticorps Recombinant de lapin anti-Band 3/AE 1

Band 3/AE 1 Recombinant Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3K21

N° de cat : 81066-1-PBS

Synonymes

Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1

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Galerie de données de validation

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  IHC staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81066-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81066-1-RR against Human Band 3/AE 1 were performed. The affinity constant is 23.6 pM.

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Informations sur le produit

81066-1-PBS cible Band 3/AE 1 dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Band 3/AE 1 Protéine recombinante Ag27878
• Nom complet: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Masse moléculaire calculée: 911 aa, 102 kDa
• Numéro d’acquisition GenBank: BC101570
• Symbole du gène: band 3
• Identification du gène (NCBI): 6521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

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