Anticorps Polyclonal de lapin anti-ATG14/Barkor (N-terminal)

• Phare
• Validé par KD/KO

ATG14/Barkor (N-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(37)

Avis (1)

N° de cat : 19491-1-AP

Synonymes

Barkor, ATG14, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Atg14L

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Galerie de données de validation

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  WB analysis of HEK-293 using 19491-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19491-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 19491-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 19491-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 19491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 19491-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules NIH/3T3, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 35 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

19491-1-AP cible ATG14/Barkor (N-terminal) dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: KIAA0831
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 65-68 kDa
• Numéro d’acquisition GenBank: NM_014924
• Symbole du gène: ATG14
• Identification du gène (NCBI): 22863
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Autophagy; Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.; Authors - Xu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (01-25-2018)

I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: U2OS

ATG14/Barkor (N-terminal) Antibody Immunofluorescence, validation (1:100 dilution) in U2OS (Cat no:19491-1-AP)

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