Anticorps Monoclonal anti-Bcl-XL

Bcl-XL Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

CloneNo.

4C12A6

N° de cat : 66020-1-Ig

Synonymes

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL

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Galerie de données de validation

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  WB analysis of Jurkat using 66020-1-Ig

  Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66020-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66020-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66020-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 66020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66020-1-Ig

  Immunofluorescent analysis of Hela cells, using BCL2L1 antibody 66020-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 66020-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HeLa, cellules K-562, cellules Ramos, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon humain, tissu de lymphome humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:200-1:600
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66020-1-Ig cible Bcl-XL dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Bcl-XL Protéine recombinante Ag18037
• Nom complet: BCL2-like 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC019307
• Symbole du gène: BCL2L1
• Identification du gène (NCBI): 598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Publications

Species	Application	Title
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
human	WB	J Immunother Cancer; Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells; Authors - Songbo Zhao
human	WB	Acta Pharmacol Sin; Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells; Authors - Wa Ding
human	WB	Blood Cancer J; The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML.; Authors - Xinan Qiao
human	WB	Antioxid Redox Signal; MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.; Authors - Tingting Ren
human	WB	J Med Chem; Design, Synthesis, and Activity Evaluation of Novel Acyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo.; Authors - Er-Jun Hao

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