Anticorps Recombinant de lapin anti-BCL2L1

• Phare
• Validé par KD/KO

BCL2L1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E8

N° de cat : 83330-1-PBS

Synonymes

Bcl-xL, 1E8, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1

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Galerie de données de validation

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  WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

  WB result of Bcl-XL antibody (83330-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bcl-XL transfected HeLa cells. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83330-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83330-1-RR against Human Bcl-XL were performed. The affinity constant is 0.792 nM.

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Informations sur le produit

83330-1-PBS cible BCL2L1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCL2L1 Protéine recombinante Ag25604
• Nom complet: BCL2-like 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC019307
• Symbole du gène: BCL2L1
• Identification du gène (NCBI): 598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.

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