Anticorps Polyclonal de lapin anti-Bcl2

• Phare
• Validé par KD/KO

Bcl2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (7)

Applications

WB, IHC, IF, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(1118)

Avis (3)

N° de cat : 26593-1-AP

Synonymes

Bcl-2, B cell leukemia/lymphoma 2, Bcl 2

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Galerie de données de validation

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  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26593-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 26593-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 26593-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 26593-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 26593-1-AP (Bcl2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NIH/3T3 using 26593-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Polyclonal antibody (26593-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C2C12, tissu cardiaque de souris, tissu cérébral de souris, tissu de côlon de souris, tissu rénal de rat, tissu rénal de souris, tissu splénique de rat, tissu splénique de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 1068 publications below
• IHC: See 62 publications below
• IF: See 23 publications below
• CoIP: See 1 publications below

Informations sur le produit

26593-1-AP cible Bcl2 dans les applications de WB, IHC, IF, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, bovin, canin, Lapin, porc, poulet, souris, Hamster, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Bcl2 Protéine recombinante Ag24260
• Nom complet: B-cell leukemia/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-009741
• Symbole du gène: Bcl2
• Identification du gène (NCBI): 12043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

   Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
   

2. What band size should I expect in western blotting for Bcl-2?

   The molecular weight of Bcl-2 is 26 kDa.

Publications

Species	Application	Title
		ACS Nano; Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway; Authors - Xirui Xin
mouse	WB	ACS Nano; Dual Strategies Based on Golgi Apparatus/Endoplasmic Reticulum Targeting and Anchoring for High-Efficiency siRNA Delivery and Tumor RNAi Therapy; Authors - Yashi Wang ; KD Validated
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
		Cell Rep Med; Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity; Authors - Xingyu Zhou
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
mouse	WB	Redox Biol; Protective role of vitamin D receptor against mitochondrial calcium overload from PM2.5-Induced injury in renal tubular cells; Authors - Mengqiu Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (08-15-2024)

The fainter band at the bottom of the membrane corresponds to the expected MW. Done in milk

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: NIH-3T3

Bcl2 Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:26593-1-AP)

FH

Anna (Verified Customer) (10-04-2022)

Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 500
• Cell Tissue Type: brain frozen sections - mouse

FH

PRADEEP (Verified Customer) (11-03-2020)

Giving very good result for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse muscle

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