Anticorps Monoclonal anti-Bcl2

Bcl2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5B9

N° de cat : 66799-1-PBS

Synonymes

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 1B5B9

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Galerie de données de validation

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  WB analysis using 66799-1-Ig (same clone as 66799-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66799-1-Ig (same clone as 66799-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66799-1-Ig (same clone as 66799-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 66799-1-Ig (same clone as 66799-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (66799-1-Ig, Clone: 1B5B9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66799-1-PBS cible Bcl2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Bcl2 Protéine recombinante Ag24261
• Nom complet: B-cell leukemia/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-009741
• Symbole du gène: Bcl2
• Identification du gène (NCBI): 12043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

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