Anticorps Monoclonal anti-Bcl2

• Phare
• Validé par KD/KO

Bcl2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3F7

N° de cat : 68103-1-PBS

Synonymes

Bcl-2, 1B3F7, B cell leukemia/lymphoma 2, Bcl 2

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Galerie de données de validation

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  WB analysis using 68103-1-Ig (same clone as 68103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 68103-1-Ig (same clone as 68103-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C2C12 using 68103-1-Ig (same clone as 68103-1-PBS)

  C2C12 cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68103-1-Ig (same clone as 68103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 68103-1-Ig (same clone as 68103-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68103-1-PBS cible Bcl2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Bcl2 Protéine recombinante Ag24261
• Nom complet: B-cell leukemia/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-009741
• Symbole du gène: Bcl2
• Identification du gène (NCBI): 12043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

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