Anticorps Recombinant de lapin anti-Bcl2

Bcl2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

240571H5

N° de cat : 82469-6-RR

Synonymes

Bcl-2, 240571H5, B cell leukemia/lymphoma 2, Bcl 2

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Galerie de données de validation

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  WB analysis of mouse spleen using 82469-6-RR

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82469-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82469-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 82469-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82469-6-RR (Bcl2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 82469-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82469-6-RR (Bcl2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of NIH/3T3 using 82469-6-RR

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Recombinant antibody (82469-6-RR, Clone:240571H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82469-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82469-6-RR against Mouse Bcl2 were performed. The affinity constant is 1.08 nM.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu cardiaque de souris, tissu cérébral de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

82469-6-RR cible Bcl2 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Bcl2 Protéine recombinante Ag24260
• Nom complet: B-cell leukemia/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-009741
• Symbole du gène: Bcl2
• Identification du gène (NCBI): 12043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Publications

Species	Application	Title
human	WB	BMC Vet Res; Cecropin AD ameliorates pneumonia and intestinal injury in mice with mycoplasma pneumoniae by mediating gut microbiota; Authors - Bowen Li
mouse	WB	ACS Nano; A Hybrid Manganese Nanoparticle Simultaneously Eliminates Cancer Stem Cells and Activates STING Pathway to Potentiate Cancer Immunotherapy; Authors - Yaping Wu

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